Micro PIV-LIF System

Micro PIV-LIF System

The Micro PIV-LIF System is designed to analyze the velocity distribution, mixing and diffusion of a microfludic system. The Micro PIV-LIF System is widely used for the development of microbio / microchemical chips called micro-TAS or labs-on-a-chip.

Various new micro PIV analyzing technologies have been adopted for the system, including average correlation and SAT-PTV.
The broad lineup of the Micro PIV System supports research and development of microfluidics technology.

Micro PIV

In addition to conventional double pulse type micro PIV (2D, Stereo 3D) and Time Resolved PIV, we provide a broad lineup of micro PIV such as confocal scanning micro PIV.
As specialized micro-optical systems, models for high-output pulse lasers or UV lasers are also included in the series.
We provide many options of peripheral devices. For example, the focus scanner can synchronise the focal plane with the frame of a high-speed video camera to permit high-speed scanning.
We also have various options for the fluorescent tracer particles necessary for micro PIV and users can choose the one most suitable for their measurements based on color, size and the characteristic of their fluid.


The velocity distribution in a 100 μm
Microchannel by confocal scanning Micro PIV

Data: Oshima Laboratory
(Institute of Industrial Science, The University of Tokyo)

Micro LIF

The micro PIV can measure concentration distribution, temperature distribution, diffusion, mixing, reaction, pH and other characteristics.
Coaxial episcopic illumination with a high-output pulse laser, which is beyond the power of general microscopes, can be used with the specialized micro-optical system.


The velocity distribution in a 100 μm
Microchannel by confocal scanning Micro PIV

Data: Hishida Sato Laboratory (Keio University)

High speed Confocal imaging

Why confocal imaging is effective in micro imaging

In micro PIV, the important concept is not Depth of Field (DOF), but Measurement Depth (MD). To define the resolution in the depth direction (the ""z resolution""). MD, which was proposed by Professor D. Meinhart (University of California, Santa Barbara) and other researchers, defines the range in which the light intensity of particle images is strong enough to affect the velocity measurement. Generally, MD is considerably larger than DOF.

In normal Micro PIV, MD is considerably thicker than the supposed DOF of the object lens, therefore, multiple velocity components included in the thickness of the MD cause errors (Fig). By contrast, in a confocal scanning micro PIV, MD can be thin enough to include only one velocity component, and therefore highly precise measurement distribution in microfluidics is possible.

Ordinarily, fluorescent particles are not used in a Micro PIV, instead a liquid that has a fluorescent agent dissolved in it is used. Consequently, the liquid becomes fluorescent in of itself. If the liquid is observed using a normal microscope, even though a high NA lens may be used, unintended stray light caused by the fluorescence in front and behind the focal plane makes spatial resolution along the optical axial (Z) deteriorate.
(Compared to the X and Y directions, deterioration of the resolution in the Z direction is more remarkable.) Confocal imaging technology cuts almost all of the fluorescence in front and behind the focal plane, therefore, the resolution in the Z direction become as high as that of X and Y directions.

 

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The bright field image  The confocal image   The principle of the confocal scanner  Confocal scanner
The bright field image  The confocal image The principle of
the confocal scanner
 
 Confocal scanner

An example of a system configuration

2D Micro PIV-LIF (Double pulse type)

microPIV
  • Double shutter type PIV camera pco.1600 1600×1200 pixel 30 fps
  • Double pulse laser 15 mJ/pulse 15 Hz 532 nm
  • Timing controller TT1680
  • Micro optical system UFS-200 Fiberbundle delivery type
  • Software for system control and data analysis: Koncerto 2D
  • PC

Stereo 3D Micro PIV-LIF (Double pulse type)

microPIV
  • Double shutter type PIV camera pco.1600 1600×1200 pixel 30 fps
  • Double pulse laser 15 mJ/pulse 15 Hz 532 nm
  • Timing controller TT1680
  • Micro optical system UFS-200 Fiberbundle delivery type
  • Calibration target
  • Software for system control and data analysis: Koncerto 2D
  • PC

Confocal scanning micro PIV-LIF

microPIV
  • Super-high-sensitivity, high-speed video camera Mi2000 512×512 pixel 2000 fps
  • Confocal scanner CSU-X1
  • CW DPSS laser 100 mW 488 nm
  • Focus scanner FS-100 (option)
  • Micro optical system UFS-200 Fiberbundle delivery type
  • Software for system control and data analysis: Koncerto 2D
  • PC

Micro PIV camera

There are double pulse type for micro PIV camera and ultra high sensitivity high speed type for confocal imaging.
Koncerto also supports other cameras than the following. Please confirm the camera correspondence situation

You can see by side scrolling.

Double pulse type


Model  pco.1600  pco.2000  pco.1200hs
resolution  1600×1200  2048×2048 1280×1024
Max frame rate 30fps  14.7fps  636fps @1280×1024
1357fps @640×480
Dynamic range   14bit  14bit 10bit 
 Min⊿t  150ns  180ns 75ns 
 cooling  ⊿-50℃  ⊿-50℃  -
Lens mount  C mount   C mount  C mount 
Image pickup device  CCD CCD  CMOS

You can see by side scrolling.

Ultrahigh sensitivity
high speed type


Model Mi2000 Mi4000 Mi8000
Maximum resolution (pixel)  512×512  512×512 1024×1024
frame rate 60 ~ 2000fps 60 ~ 4000fps 60 ~ 8000fps
frame rate (fps)
@ resolution (pixel)
2000fps 2000fps @512×512
4000fps @512×256
1000fps @1000×1000
4000fps @512×512
8000fps @512×256
Memory capacity 2.6GB 2.6GB 2GB (maximum 16GB)
Number of frames 8,192 @512×512 8,192 @512×512  -
Dynamic range 10 bits (monochrome)
Lens mount  C mount
Shutter system Electronic shutter
Image intensifier GEN-Ⅲ type (high sensitivity GaAsP photocathode)
Image pickup device CMOS

 

横スクロールでご覧いただけます。

Why confocal imaging is effective in micro imaging?

In micro PIV, the important concept is not Depth of Field (DOF), but Measurement Depth (MD).
To define the resolution in the depth direction (the ""z resolution"").
MD, which was proposed by Professor D. Meinhart (University of California, Santa Barbara) and other researchers, defines the range in which the light intensity of particle images is strong enough to affect the velocity measurement.
Generally, MD is considerably larger than DOF.

In normal Micro PIV, MD is considerably thicker than the supposed DOF of the object lens, therefore, multiple velocity components included in the thickness of the MD cause errors (Fig).
By contrast, in a confocal scanning micro PIV, MD can be thin enough to include only one velocity component, and therefore highly precise measurement distribution in microfluidics is possible.

Ordinarily, fluorescent particles are not used in a Micro PIV, instead a liquid that has a fluorescent agent dissolved in it is used. Consequently, the liquid becomes fluorescent in of itself.
If the liquid is observed using a normal microscope, even though a high NA lens may be used, unintended stray light caused by the fluorescence in front and behind the focal plane makes spatial resolution along the optical axial (Z) deteriorate.
(Compared to the X and Y directions, deterioration of the resolution in the Z direction is more remarkable.)
Confocal imaging technology cuts almost all of the fluorescence in front and behind the focal plane, therefore, the resolution in the Z direction become as high as that of X and Y directions.

 
 

The bright field image
 

 The confocal image



The principle of
the confocal scanner 



 Confocal scanner





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